Open Access Articles- Top Results for K%2B-ATPase


File:Sodium Pump.svg
Alpha and beta units.
Sodium-potassium pump, E2-Pi state. Calculated hydrocarbon boundaries of the lipid bilayer are shown as blue (intracellular) and red (extracellular) planes

Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase (sodium-potassium adenosine triphosphatase, also known as Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom pump, sodium-potassium pump, or sodium pump) is an antiporter-like enzyme (EC (an electrogenic transmembrane ATPase) located in the plasma membrane of all animal cells. The Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase enzyme pumps sodium out of cells, while pumping potassium into cells. It has antiporter-like activity but is not actually an antiporter since both molecules are moving against their concentration gradient.

Sodium-potassium pumps

Active transport is responsible for the fact that cells contain a relatively high concentrations of potassium ions but low concentrations of sodium ions. The mechanism responsible for this is the sodium-potassium pump, which moves these two ions in opposite directions across the plasma membrane. This was investigated by following the passage of radioactively labeled ions across the plasma membrane of certain cells. It was found that the concentrations of sodium and potassium ions on the two sides of the membrane are interdependent, suggesting that the same carrier transports both ions. It is now known that the carrier is an ATP-ase and that it pumps three sodium ions out of the cell for every two potassium ions pumped in.

The sodium-potassium pump was discovered in the 1950s by a Danish scientist, Jens Christian Skou, who was awarded a Nobel Prize in 1997. It marked an important step forward in the understanding of how ions get into and out of cells, and it has a particular significance for excitable cells such as nervous cells, which depend on this pump for responding to stimuli and transmitting impulses.


The Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase helps maintain resting potential, effect transport, and regulate cellular volume.[1] It also functions as a signal transducer/integrator to regulate MAPK pathway, ROS, as well as intracellular calcium. In most animal cells, the Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase is responsible for about 1/5 of the cell's energy expenditure.[citation needed] For neurons, the Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase can be responsible for up to 2/3 of the cell's energy expenditure.[2]

Resting potential

File:Sodium-potassium pump and diffusion.png
The Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase, as well as effects of diffusion of the involved ions maintain the resting potential across the membranes.

In order to maintain the cell membrane potential, cells keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). The sodium-potassium pump moves 3 sodium ions out and moves 2 potassium ions in, thus, in total, removing one positive charge carrier from the intracellular space. (Please see Mechanism for details).

The action of the sodium-potassium pump is not the only mechanism responsible for the generation of the resting membrane potential. Also, the selective permeability of the cell's plasma membrane for the different ions plays an important role. All mechanisms involved are explained in the main article on generation of the resting membrane potential.


Export of sodium from the cell provides the driving force for several secondary active transporters membrane transport proteins, which import glucose, amino acids, and other nutrients into the cell by use of the sodium gradient.

Another important task of the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump is to provide a [[Sodium|Template:Chem/atomTemplate:Chem/atom]] gradient that is used by certain carrier processes. In the gut, for example, sodium is transported out of the reabsorbing cell on the blood (interstitial fluid) side via the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump, whereas, on the reabsorbing (lumenal) side, the Template:Chem/atomTemplate:Chem/atom-glucose symporter uses the created Template:Chem/atomTemplate:Chem/atom gradient as a source of energy to import both Template:Chem/atomTemplate:Chem/atom and glucose, which is far more efficient than simple diffusion. Similar processes are located in the renal tubular system.

Controlling cell volume

Failure of the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pumps can result in swelling of the cell. A cell's osmolarity is the sum of the concentrations of the various ion species and many proteins and other organic compounds inside the cell. When this is higher than the osmolarity outside of the cell, water flows into the cell through osmosis. This can cause the cell to swell up and lyse. The Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump helps to maintain the right concentrations of ions. Furthermore, when the cell begins to swell, this automatically activates the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump.[citation needed]

Functioning as signal transducer

Within the last decade [when?], many independent labs have demonstrated that, in addition to the classical ion transporting, this membrane protein can also relay extracellular ouabain-binding signalling into the cell through regulation of protein tyrosine phosphorylation. The downstream signals through ouabain-triggered protein phosphorylation events include activation of the mitogen-activated protein kinase (MAPK) signal cascades, mitochondrial reactive oxygen species (ROS) production, as well as activation of phospholipase C (PLC) and inositol triphosphate (IP3) receptor (IP3R) in different intracellular compartments.[3]

Protein-protein interactions play a very important role in Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump-mediated signal transduction. For example, Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump interacts directly with Src, a non-receptor tyrosine kinase, to form a signaling receptor complex.[4] Src kinase is inhibited by Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump, while, upon ouabain binding, the Src kinase domain will be released and then activated. Based on this scenario, NaKtide, a peptide Src inhibitor derived from Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump, was developed as a functional ouabain-Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump-mediated signal transduction.[5] Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump also interacts with ankyrin, IP3R, PI3K, PLC-gamma and cofilin.[6]

Controlling neuron activity states

The Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump has been shown to control and set the intrinsic activity mode of cerebellar Purkinje neurons.[7] This suggests that the pump might not simply be a homeostatic, "housekeeping" molecule for ionic gradients; but could be a computation element in the cerebellum and the brain.[8] Indeed, a mutation in the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump causes rapid onset dystonia parkinsonism, which has symptoms to indicate that it is a pathology of cerebellar computation.[9] Furthermore, an ouabain block of Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pumps in the cerebellum of a live mouse results in it displaying ataxia and dystonia.[10] The distribution of the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump on myelinated axons, in human brain, was demonstrated to be along the internodal axolemma, and not within the nodal axolemma as previously thought.[11]




The Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase is upregulated by cAMP.[12] Thus, substances causing an increase in cAMP upregulate the Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase. These include the ligands of the Gs-coupled GPCRs. In contrast, substances causing a decrease in cAMP downregulate the Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase. These include the ligands of the Gi-coupled GPCRs.

Note: Early studies indicated the opposite effect, but these were later found to be inaccurate due to additional complicating factors.[citation needed]


The Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom-ATPase can be pharmacologically modified by administrating drugs exogenously.

For instance, Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom-ATPase found in the membrane of heart cells is an important target of cardiac glycosides (for example digoxin and ouabain), inotropic drugs used to improve heart performance by increasing its force of contraction.

Muscle contraction is dependent on a 100- to 10,000-times-higher-than-resting intracellular [[calcium in biology|Template:Chem/atomTemplate:Chem/atom]] concentration, which is caused by Template:Chem/atomTemplate:Chem/atom release from the muscle cells' sarcoplasmic reticulum. Immediately after muscle contraction, intracellular Template:Chem/atomTemplate:Chem/atom is quickly returned to its normal concentration by a carrier enzyme in the plasma membrane, and a calcium pump in sarcoplasmic reticulum, causing the muscle to relax.

Since this carrier enzyme (Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom translocator) uses the Na gradient generated by the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump to remove Template:Chem/atomTemplate:Chem/atom from the intracellular space, slowing down the Template:Chem/atomTemplate:Chem/atom-Template:Chem/atomTemplate:Chem/atom pump results in a permanently elevated Template:Chem/atomTemplate:Chem/atom level in the muscle, which may be the mechanism of the long-term inotropic effect of cardiac glycosides such as digoxin.


Template:Chem/atomTemplate:Chem/atomTemplate:Chem/atomTemplate:Chem/atom-ATPase was discovered by Jens Christian Skou in 1957 while working as assistant professor at the Department of Physiology, University of Aarhus, Denmark. He published his work that year.[13]

In 1997, he received one-half of the Nobel Prize in Chemistry "for the first discovery of an ion-transporting enzyme, Template:Chem/atomTemplate:Chem/atom, Template:Chem/atomTemplate:Chem/atom -ATPase."[14]


See also


  1. ^ a b Hall, John E.; Guyton, Arthur C. (2006). Textbook of medical physiology. St. Louis, Mo: Elsevier Saunders. ISBN 0-7216-0240-1. 
  2. ^ Howarth C, Gleeson P, Attwell D (July 2012). "Updated energy budgets for neural computation in the neocortex and cerebellum". J. Cereb. Blood Flow Metab. 32 (7): 1222–32. PMID 22434069. doi:10.1038/jcbfm.2012.35. 
  3. ^ Yuan Z, Cai T, Tian J, Ivanov AV, Giovannucci DR, Xie Z (September 2005). "Na/K-ATPase tethers phospholipase C and IP3 receptor into a calcium-regulatory complex". Molecular Biology of the Cell 16 (9): 4034–45. PMC 1196317. PMID 15975899. doi:10.1091/mbc.E05-04-0295. 
  4. ^ Tian J, Cai T, Yuan Z et al. (January 2006). "Binding of Src to Na+/K+-ATPase forms a functional signaling complex". Molecular Biology of the Cell 17 (1): 317–26. PMC 1345669. PMID 16267270. doi:10.1091/mbc.E05-08-0735. 
  5. ^ Li Z, Cai T, Tian J et al. (July 2009). "NaKtide, a Na/K-ATPase-derived peptide Src inhibitor, antagonizes ouabain-activated signal transduction in cultured cells". The Journal of Biological Chemistry 284 (31): 21066–76. PMC 2742871. PMID 19506077. doi:10.1074/jbc.M109.013821. 
  6. ^ Lee K, Jung J, Kim M, Guidotti G (January 2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". The Biochemical Journal 353 (2): 377–85. PMC 1221581. PMID 11139403. doi:10.1042/0264-6021:3530377. 
  7. ^ Forrest MD, Wall MJ, Press DA, Feng J (December 2012). "The Sodium-Potassium Pump Controls the Intrinsic Firing of the Cerebellar Purkinje Neuron". PLoS ONE 7 (12): e51169. PMC 3527461. PMID 23284664. doi:10.1371/journal.pone.0051169. 
  8. ^ Forrest MD (December 2014). "The sodium-potassium pump is an information processing element in brain computation". Frontiers in Physiology 5 (472). doi:10.3389/fphys.2014.00472. 
  9. ^ Cannon C (July 2004). "Paying the Price at the Pump: Dystonia from Mutations in a Na+/K+-ATPase". Neuron 43 (2): 153–154. PMID 15260948. doi:10.1016/j.neuron.2004.07.002. 
  10. ^ Calderon DP, Fremont R, Kraenzlin F, Khodakhah K (March 2011). "The neural substrates of rapid-onset Dystonia-Parkinsonism". Nature Neuroscience 14 (3): 357–65. PMC 3430603. PMID 21297628. doi:10.1038/nn.2753. 
  11. ^ Young EA, Fowler CD, Kidd GJ, Chang A, Rudick R, Fisher E, Trapp BD (April 2008). "Imaging correlates of decreased axonal Na+/K+ ATPase in chronic multiple sclerosis lesions.". Ann Neurol 63 (4): 428–35. PMID 18438950. doi:10.1002/ana.21381. 
  12. ^ Burnier, Michel (2008). Sodium In Health And Disease. CRC Press. p. 15. ISBN 978-0-8493-3978-3. 
  13. ^ Skou JC (February 1957). "The influence of some cations on an adenosine triphosphatase from peripheral nerves". Biochimica et Biophysica Acta 23 (2): 394–401. PMID 13412736. doi:10.1016/0006-3002(57)90343-8. 
  14. ^ Chemistry 1997

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