SymbolsABCB1 ; ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1
External IDsOMIM171050 MGI97570 HomoloGene55496 IUPHAR: 768 ChEMBL: 4302 GeneCards: ABCB1 Gene
EC number3.6.3.44
RNA expression pattern
File:PBB GE ABCB1 209994 s at tn.png
File:PBB GE ABCB1 209993 at tn.png
More reference expression data
RefSeq (mRNA)NM_000927NM_011076
RefSeq (protein)NP_000918NP_035206
Location (UCSC)Chr 7:
87.13 – 87.34 Mb
Chr 5:
8.66 – 8.75 Mb
PubMed search[1][2]
ABCB1 is differentially expressed in 97 experiments [93 up/106 dn]: 26 organism parts: kidney [2 up/0 dn], bone marrow [0 up/2 dn], ...; 29 disease states: normal [10 up/3 dn], glioblastoma [0 up/2 dn], ...; 30 cell types, 22 cell lines, 11 compound treatments and 16 other conditions.
Factor Value Factor Up/Down
Legend: File:Atlas-legend.png - number of studies the gene is up/down in
Normal Disease state 10/3
None Compound treatment 3/0
Stromal cell Cell type 1/2
Kidney Cell type 2/0
MDA-MB-231 Cell line 0/2
Glioblastoma Disease state 0/2
Epithelial cell Cell type 0/2
HeLa Cell line 0/2
Primary Disease staging 2/0
Bone marrow Organism part 0/2
ABCB1 expression data in ATLAS

P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi and bacteria and likely evolved as a defense mechanism against harmful substances.

P-gp is extensively distributed and expressed in the intestinal epithelium where it pumps xenobiotics (such as toxins or drugs) back into the intestinal lumen, in liver cells where it pumps them into bile ducts, in the cells of the proximal tubule of the kidney where it pumps them into urine-conducting ducts, and in the capillary endothelial cells composing the blood–brain barrier and blood-testis barrier, where it pumps them back into the capillaries. Some cancer cells also express large amounts of P-gp, which renders these cancers multi-drug resistant.

P-gp is a glycoprotein that in humans is encoded by the ABCB1 gene.[1] P-gp is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily.[2]

P-gp was discovered in 1971 by Victor Ling.


The protein belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. P-gp is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.[3]

P-gp transports various substrates across the cell membrane including:

Its ability to transport the above substrates accounts for the many roles of P-gp including:

  • Regulating the distribution and bioavailability of drugs
    • Increased intestinal expression of P-glycoprotein can reduce the absorption of drugs that are substrates for P-glycoprotein. Thus, there is a reduced bioavailability, and therapeutic plasma concentrations are not attained. On the other hand, supratherapeutic plasma concentrations and drug toxicity may result because of decreased P-glycoprotein expression
    • Active cellular transport of antineoplastics resulting in multidrug resistance to these drugs
  • The removal of toxic metabolites and xenobiotics from cells into urine, bile, and the intestinal lumen
  • The transport of compounds out of the brain across the blood–brain barrier
  • Digoxin uptake
  • Prevention of ivermectin and loperamide entry into the central nervous system
  • The migration of dendritic cells
  • Protection of hematopoietic stem cells from toxins.[2]

It is inhibited by many drugs, such as:[4]


P-gp is a 170 kDa transmembrane glycoprotein, which includes 10-15 kDa of N-terminal glycosylation. The N-terminal half of the molecule contains 6 transmembrane domains, followed by a large cytoplasmic domain with an ATP-binding site, and then a second section with 6 transmembrane domains and an ATP-binding site that shows over 65% of amino acid similarity with the first half of the polypeptide.[5] In 2009, the first structure of a mammalian P-glycoprotein was solved (3G5U).[6] The structure was derived from the mouse MDR3 gene product heterologously expressed in Pichia pastoris yeast. The structure of mouse P-gp is similar to structures of the bacterial ABC transporter MsbA (3B5W and 3B5X)[7] that adopt an inward facing conformation that is believed to be important for binding substrate along the inner leaflet of the membrane. Additional structures (3G60 and 3G61) of P-gp were also solved revealing the binding site(s) of two different cyclic peptide substrate/inhibitors. The promiscuous binding pocket of P-gp is lined with aromatic amino acid side chains. However, the murine P-gp structure is incomplete, missing an intermediate linker sequence proved to be essential for substrate recognition and ATP hydrolysis. Through Molecular Dynamic (MD) simulations, this sequence was proved to have a direct impact in the transporter's structural stability (in the nucleotide-binding domains) and defining a lower boundary for the internal drug-binding pocket.[8]

Mechanism of action

Substrate enters P-gp either from an opening within the inner leaflet of the membrane or from an opening at the cytoplasmic side of the protein. ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a position to be excreted from the cell. Release of the phosphate (from the original ATP molecule) occurs concurrently with substrate excretion. ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a phosphate molecule resets the protein, so that the process can start again.

Tissue distribution

P-gp is expressed primarily in certain cell types in the liver, pancreas, kidney, colon, and jejunum.[9] P-gp is also found in brain capillary endothelial cells.[10]

Detecting the activity of the transporter

The activity of the transporter can be determined by both membrane ATPase and cellular calcein assays.

Radioactive verapamil can be used for measuring P-gp function with positron emission tomography.[11]

P-gp is also used to differentiate transitional B-cells from naive B-cells. Dyes such as Rhodamine123 and MitoTracker Dyes from Invitrogen can be used to make this differentiation.[12]


P-gp was first cloned and characterized in 1976. It was shown to be responsible for conferring multidrug resistance upon mutant cultured cancer cells that had developed resistance to cytotoxic drugs.[2][13]

The structure of P-gp was resolved by x-ray crystallography in 2009.[6]


  1. ^ Ueda K, Clark DP, Chen CJ, Roninson IB, Gottesman MM, Pastan I (January 1987). "The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation". J. Biol. Chem. 262 (2): 505–8. PMID 3027054. 
  2. ^ a b c Dean, Michael (2002-11-01). "The Human ATP-Binding Cassette (ABC) Transporter Superfamily". National Library of Medicine (US), NCBI. Retrieved 2008-03-02. 
  3. ^ "Entrez Gene: ABCB1". 
  4. ^ "Drug Development and Drug Interactions: Table of Substrates, Inhibitors and Inducers". 
  5. ^ Franck Viguié (1998-03-01). "ABCB1". Atlas of Genetics and Cytogenetics in Oncology and Haematology. Retrieved 2008-03-02. 
  6. ^ a b Stephen Aller; Jodie Yu, Andrew Ward, Yue Weng, Srinivas Chittaboina, Rupeng Zhuo, Patina M. Harrell, Yenphuong T. Trinh,Qinghai Zhang, Ina L. Urbatsch, Geoffrey Chang (2009-03-27). "Structure of P-glycoprotein Reveals a Molecular Basis for Poly-Specific Drug Binding". Science (Science) 323 (5922): 1718–1722. PMC 2720052. PMID 19325113. doi:10.1126/science.1168750. Retrieved 2009-04-12. 
  7. ^ Ward A, Reyes CL, Yu J, Roth CB, Chang G (November 2007). "Flexibility in the ABC transporter MsbA: Alternating access with a twist". Proc. Natl. Acad. Sci. U.S.A. 104 (48): 19005–10. PMC 2141898. PMID 18024585. doi:10.1073/pnas.0709388104. 
  8. ^ Ferreira RJ, Ferreira M-JU, dos Santos DJVA (May 2012). "Insights on P-Glycoprotein’s Efflux Mechanism obtained by Molecular Dynamics Simulations". J Chem Theory Comput 8 (6): 1853–1864. doi:10.1021/ct300083m. 
  9. ^ Thiebaut F, Tsuruo T, Hamada H, Gottesman MM, Pastan I, Willingham MC (November 1987). "Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues". Proc. Natl. Acad. Sci. U.S.A. 84 (21): 7735–8. PMC 299375. PMID 2444983. doi:10.1073/pnas.84.21.7735. 
  10. ^ Schinkel AH (April 1999). "P-Glycoprotein, a gatekeeper in the blood–brain barrier". Advanced Drug Delivery Reviews 5 (36(2-3)): 179–194. PMID 10837715. doi:10.1016/S0169-409X(98)00085-4. 
  11. ^ Luurtsema G, Windhorst AD, Mooijer MPJ, Herscheid A, Lammertsma AA, Franssen EJF (2002). "Fully automated high yield synthesis of (R)- and (S)-[C-11]verapamil for measuring P-glycoprotein function with positron emission tomography". Journal of Labelled Compounds & Radiopharmaceuticals 45 (14): 1199–1207. doi:10.1002/jlcr.632. 
  12. ^ Wirths S, Lanzavecchia A (December 2005). "ABCB1 transporter discriminates human resting naive B cells from cycling transitional and memory B cells". Eur. J. Immunol. 35 (12): 3433–41. PMID 16259010. doi:10.1002/eji.200535364. 
  13. ^ Juliano RL, Ling V (1976). "A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants". Biochim. Biophys. Acta 455 (1): 152–62. PMID 990323. doi:10.1016/0005-2736(76)90160-7. 

Further reading

  • Ling V (1997). "Multidrug resistance: molecular mechanisms and clinical relevance". Cancer Chemother. Pharmacol. 40 Suppl (7): S3–8. PMID 9272126. doi:10.1007/s002800051053. 
  • Kerb R, Hoffmeyer S, Brinkmann U (2001). "ABC drug transporters: hereditary polymorphisms and pharmacological impact in MDR1, MRP1 and MRP2". Pharmacogenomics 2 (1): 51–64. PMID 11258197. doi:10.1517/14622416.2.1.51. 
  • Akiyama S (2002). "[Mechanisms of drug resistance and reversal of the resistance]". Hum. Cell 14 (4): 257–60. PMID 11925925. 
  • Brinkmann U (2002). "Functional polymorphisms of the human multidrug resistance (MDR1) gene: correlation with P glycoprotein expression and activity in vivo". Novartis Found. Symp. Novartis Foundation Symposia 243: 207–10; discussion 210–2, 231–5. ISBN 978-0-470-84635-3. PMID 11990778. doi:10.1002/0470846356.ch15. 
  • Váradi A, Szakács G, Bakos E, Sarkadi B (2002). "P glycoprotein and the mechanism of multidrug resistance". Novartis Found. Symp. Novartis Foundation Symposia 243: 54–65; discussion 65–8, 180–5. ISBN 978-0-470-84635-3. PMID 11990782. doi:10.1002/0470846356.ch5. 
  • Hegedus T, Orfi L, Seprodi A et al. (2002). "Interaction of tyrosine kinase inhibitors with the human multidrug transporter proteins, MDR1 and MRP1". Biochim. Biophys. Acta 1587 (2–3): 318–25. PMID 12084474. doi:10.1016/s0925-4439(02)00095-9. 
  • Pallis M, Turzanski J, Higashi Y, Russell N (2003). "P-glycoprotein in acute myeloid leukaemia: therapeutic implications of its association with both a multidrug-resistant and an apoptosis-resistant phenotype". Leuk. Lymphoma 43 (6): 1221–8. PMID 12152989. doi:10.1080/10428190290026277. 
  • Schaich M, Illmer T (2003). "Mdr1 gene expression and mutations in Ras proto-oncogenes in acute myeloid leukemia". Leuk. Lymphoma 43 (7): 1345–54. PMID 12389613. doi:10.1080/10428190290033279. 
  • Fromm MF (2003). "The influence of MDR1 polymorphisms on P-glycoprotein expression and function in humans". Adv. Drug Deliv. Rev. 54 (10): 1295–310. PMID 12406646. doi:10.1016/S0169-409X(02)00064-9. 
  • Ambudkar SV, Kimchi-Sarfaty C, Sauna ZE, Gottesman MM (2003). "P-glycoprotein: from genomics to mechanism". Oncogene 22 (47): 7468–85. PMID 14576852. doi:10.1038/sj.onc.1206948. 
  • Jamroziak K, Robak T (2004). "Pharmacogenomics of MDR1/ABCB1 gene: the influence on risk and clinical outcome of haematological malignancies". Hematology 9 (2): 91–105. PMID 15203864. doi:10.1080/10245330310001638974. 
  • Ishikawa T, Onishi Y, Hirano H et al. (2005). "Pharmacogenomics of drug transporters: a new approach to functional analysis of the genetic polymorphisms of ABCB1 (P-glycoprotein/MDR1)". Biol. Pharm. Bull. 27 (7): 939–48. PMID 15256718. doi:10.1248/bpb.27.939. 
  • Lee W, Lockhart AC, Kim RB, Rothenberg ML (2005). "Cancer pharmacogenomics: powerful tools in cancer chemotherapy and drug development". Oncologist 10 (2): 104–11. PMID 15709212. doi:10.1634/theoncologist.10-2-104. 
  • Gambrelle J, Labialle S, Dayan G et al. (2005). "[Multidrug resistance in uveal melanoma.]". Journal français d'ophtalmologie 28 (6): 652–9. PMID 16141933. doi:10.1016/s0181-5512(05)81112-x. 
  • Al-Shawi MK, Omote H (2006). "The Remarkable Transport Mechanism of P-glycoprotein; a Multidrug Transporter". J. Bioenerg. Biomembr. 37 (6): 489–96. PMC 1459968. PMID 16691488. doi:10.1007/s10863-005-9497-5. 
  • Ferreira RJ, Ferreira M-JU, dos Santos DJVA (2012). "Insights on P-Glycoprotein’s Efflux Mechanism obtained by Molecular Dynamics Simulations". J Chem Theory Comput 8 (6): 1853–1864. doi:10.1021/ct300083m. 
  • Orlowski S, Martin S, Escargueil A (2006). "P-glycoprotein and 'lipid rafts': some ambiguous mutual relationships (floating on them, building them or meeting them by chance?)". Cell. Mol. Life Sci. 63 (9): 1038–59. PMID 16721513. doi:10.1007/s00018-005-5554-9. 
  • Annese V, Valvano MR, Palmieri O et al. (2006). "Multidrug resistance 1 gene in inflammatory bowel disease: a meta-analysis". World J. Gastroenterol. 12 (23): 3636–44. PMID 16773678. 
  • Sekine I, Minna JD, Nishio K et al. (2007). "A literature review of molecular markers predictive of clinical response to cytotoxic chemotherapy in patients with lung cancer". Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 1 (1): 31–7. PMID 17409824. doi:10.1097/01243894-200601000-00008. 
  • Kumar YS, Adukondalu D, Sathish D, Vishnu YV, Ramesh G, Latha AB, Reddy PC, Sarangapani M, Rao YM (2010). "P-Glycoprotein- and cytochrome P-450-mediated herbal drug interactions". Drug Metabol Drug Interact 25 (1-4): 3–16. PMID 21417789. doi:10.1515/DMDI.2010.006. 
  • Shityakov S, Förster C (2013). "Multidrug resistance protein P-gp interaction with nanoparticles (fullerenes and carbon nanotube) to assess their drug delivery potential: a theoretical molecular docking study". Int. J. Comput. Biol. Drug Des. 6 (4): 343–57. doi:10.1504/IJCBDD.2013.056801. 
  • Shityakov S, Förster C (2014). "In silico structure-based screening of versatile P-glycoprotein inhibitors using polynomial empirical scoring functions". Adv. Appl. Bioinform. Chem. 24 (7): 1–9. doi:10.2147/AABC.S56046. 
  • Schinkel AH (April 1999). "P-Glycoprotein, a gatekeeper in the blood–brain barrier". Advanced Drug Delivery Reviews 5 (36(2-3)): 179–194. PMID 10837715. 

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.