Open Access Articles- Top Results for Protein kinase C zeta type

Protein kinase C zeta type

External IDsOMIM176982 MGI97602 HomoloGene55681 IUPHAR: 1491 ChEMBL: 3438 GeneCards: PRKCZ Gene
EC number2.7.11.13
RNA expression pattern
File:PBB GE PRKCZ 202178 at tn.png
More reference expression data
RefSeq (mRNA)NM_001033581NM_001039079
RefSeq (protein)NP_001028753NP_001034168
Location (UCSC)Chr 1:
1.98 – 2.12 Mb
Chr 4:
155.26 – 155.36 Mb
PubMed search[1][2]

Protein kinase C, zeta (PKCζ), also known as PRKCZ, is an enzyme that in humans is encoded by the PRKCZ gene. The PRKCZ gene encodes at least two alternative transcripts, the full-length PKCζ and an N-terminal truncated form PKMζ. PKMζ is thought to be responsible for maintaining long-term memories in the brain. The importance of PKCζ in the creation and maintenance of long-term potentiation was first described by Todd Sacktor and his colleagues at the State University of New York at Brooklyn in 1993.[1]


PKC-zeta has an N-terminal regulatory domain, followed by a hinge region and a C-terminal catalytic domain. Second messengers stimulate PKCs by binding to the regulatory domain, translocating the enzyme from cytosol to membrane, and producing a conformational change that removes auto-inhibition of the PKC catalytic protein kinase activity. PKM-zeta, a brain-specific isoform of PKC-zeta generated from an alternative transcript, lacks the regulatory region of full-length PKC-zeta and is therefore constitutively active.[2]

PKMζ is the independent catalytic domain of PKCζ and, lacking an autoinhibitory regulatory domain of the full-length PKCζ, is constitutively and persistently active, without the need of a second messenger. It was originally thought of as being a cleavage product of full-length PKCζ, an atypical isoform of protein kinase C (PKC). Like other PKC isoforms, PKCζ is a serine/threonine kinase that adds phosphate groups to target proteins. It is atypical in that unlike other PKC isoforms, PKCζ does not require calcium or diacylglycerol (DAG) to become active, but rather relies on a different second messenger, presumably generated through a phosphoinositide 3-kinase (PI3-kinase) pathway. It is now known that PKMζ is not the result of cleavage of full-length PKCζ, but rather, in the mammalian brain, is translated from its own brain-specific mRNA, that is transcribed by an internal promoter within the PKCζ gene.[2] The promoter for full-length PKCζ is largely inactive in the forebrain and so PKMζ is the dominant form of ζ in the forebrain and the only PKM that is translated from its own mRNA.



Atypical PKC (aPKC) isoforms [zeta (this enzyme) and lambda/iota] play important roles in insulin-stimulated glucose transport. Human adipocytes contain PKC-zeta, rather than PKC-lambda/iota, as their major aPKC. Inhibition of the PKCζ enzyme inhibits insulin-stimulated glucose transport while activation of PKCζ increases glucose transport.[3]


PKMζ is thought to be responsible for maintaining the late phase of long-term potentiation (LTP);[4][5][6] However, transgenic mice lacking PKMζ demonstrate normal LTP.[7] LTP is one of the major cellular mechanisms that are widely considered to underlie learning and memory.[8] This theory arose from the observation that PKMζ perfused post synaptically into neurons causes synaptic potentiation, and selective inhibitors of PKMζ like zeta inhibitory peptide (ZIP), when bath applied one hour after tetanization, inhibit the late phase or maintenance of LTP. Thus PKMζ is both necessary and sufficient for maintaining LTP. Subsequent work showed that inhibiting PKMζ reversed LTP maintenance when applied up to 5 hours after LTP was induced in hippocampal slices, and after 22 hours in vivo. Inhibiting PKMζ in behaving animals erased spatial long-term memories in the hippocampus that were up to one month old, without affecting spatial short-term memories,[6] and erased long-term memories for fear conditioning and inhibitory avoidance in the basolateral amygdala.[9] When ZIP was injected into rats' sensorimotor cortices, it erased muscle memories for a task, even after several weeks of training.[10] In the neocortex, thought to be the site of storage for most long-term memories, PKMζ inhibition erased associative memories for conditioned taste aversion in the insular cortex, up to 3 months after training.[11][12] The protein also seems to be involved, through the nucleus accumbens, in the consolidation and reconsolidation of the memory related to drug addiction.[13] PKMζ is thus the first molecule shown to be a component of the storage mechanism of long-term memory, however this function has recently been challenged.[14][15]

Recent research has demonstrated alteration in PKMζ in Alzheimer's disease (see Long-term potentiation), providing a potential link between this kinase and neurodegeneration.[16]

Model organisms

Model organisms have been used in the study of PRKCZ function. A conditional knockout mouse line, called Prkcztm1a(EUCOMM)Wtsi[23][24] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[25][26][27]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[21][28] Twenty five tests were carried out on mutant mice and three significant abnormalities were observed.[21] Homozygous mutant males had Bergmeister's papilla, while both sexes had atypical plasma chemistry and abnormal melanocyte morphology.[21]


  • 1,3,5-Trisubstituted Pyrazolines[29]


PRKCZ has been shown to interact with:


  1. Sacktor TC, Osten P, Valsamis H, Jiang X, Naik MU, Sublette E (1993). "Persistent activation of the zeta isoform of protein kinase C in the maintenance of long-term potentiation". Proceedings of the National Academy of Sciences of the United States of America 90 (18): 8342–8346. PMC 47352. PMID 8378304. doi:10.1073/pnas.90.18.8342. 
  2. 2.0 2.1 Hernandez AI, Blace N, Crary JF, Serrano PA, Leitges M, Libien JM et al. (October 2003). "Protein kinase M zeta synthesis from a brain mRNA encoding an independent protein kinase C zeta catalytic domain. Implications for the molecular mechanism of memory". J. Biol. Chem. 278 (41): 40305–16. PMID 12857744. doi:10.1074/jbc.M307065200. 
  3. Bandyopadhyay G, Sajan MP, Kanoh Y, Standaert ML, Quon MJ, Lea-Currie R et al. (February 2002). "PKC-zeta mediates insulin effects on glucose transport in cultured preadipocyte-derived human adipocytes". J. Clin. Endocrinol. Metab. 87 (2): 716–23. PMID 11836310. doi:10.1210/jc.87.2.716. 
  4. Ling DS, Benardo LS, Serrano PA, Blace N, Kelly MT, Crary JF et al. (2002). "Protein kinase Mzeta is necessary and sufficient for LTP maintenance". Nat. Neurosci. 5 (4): 295–6. PMID 11914719. doi:10.1038/nn829. 
  5. Serrano P, Yao Y, Sacktor TC (2005). "Persistent phosphorylation by protein kinase Mzeta maintains late-phase long-term potentiation". J Neurosci 25 (8): 1979–84. PMID 15728837. doi:10.1523/JNEUROSCI.5132-04.2005. 
  6. 6.0 6.1 Pastalkova E, Serrano P, Pinkhasova D, Wallace E, Fenton AA, Sacktor TC (2006). "Storage of spatial information by the maintenance mechanism of LTP". Science 313 (5790): 1141–4. PMID 16931766. doi:10.1126/science.1128657. 
  7. Volk LJ, Bachman JL, Johnson R, Yu Y, Huganir RL (2013). "PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory". Nature 493 (7432): 420–423. PMC 3830948. PMID 23283174. doi:10.1038/nature11802. 
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  13. Crespo JA, Stöckl P, Ueberall F, Jenny M, Saria A, Zernig G (February 2012). "Activation of PKCzeta and PKMzeta in the nucleus accumbens core is necessary for the retrieval, consolidation and reconsolidation of the drug memory". PLoS ONE 7 (2): e30502. PMID 22348011. doi:10.1371/journal.pone.0030502. 
  14. Volk LJ, Bachman JL, Johnson R, Yu Y, Huganir RL (January 2013). "PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory". Nature 493 (7432): 420–3. PMC 3830948. PMID 23283174. doi:10.1038/nature11802. Retrieved 2 April 2013. 
  15. Lee AM, Kanter BR, Wang D, Lim JP, Zou ME, Qiu C et al. (January 2013). "Prkcz null mice show normal learning and memory". Nature 493 (7432): 416–9. PMC 3548047. PMID 23283171. doi:10.1038/nature11803. Retrieved 2 April 2013. 
  16. Crary JF, Shao CY, Mirra SS, Hernandez AI, Sacktor TC (April 2006). "Atypical protein kinase C in neurodegenerative disease I: PKMzeta aggregates with limbic neurofibrillary tangles and AMPA receptors in Alzheimer disease". J. Neuropathol. Exp. Neurol. 65 (4): 319–26. PMID 16691113. doi:10.1097/01.jnen.0000218442.07664.04. 
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  20. "Citrobacter infection data for Prkcz". Wellcome Trust Sanger Institute. 
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  29. Abdel-Halim et al (2014): "Discovery and Optimization of 1,3,5-Trisubstituted Pyrazolines as Potent and Highly Selective Allosteric Inhibitors of Protein Kinase C‑ζ". Journal of Medicinal Chemistry. epub.
  30. Hodgkinson CP, Sale EM, Sale GJ (2002). "Characterization of PDK2 activity against protein kinase B gamma". Biochemistry 41 (32): 10351–9. PMID 12162751. doi:10.1021/bi026065r. 
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Further reading

  • Slater SJ, Ho C, Stubbs CD (2003). "The use of fluorescent phorbol esters in studies of protein kinase C-membrane interactions". Chem. Phys. Lipids 116 (1–2): 75–91. PMID 12093536. doi:10.1016/S0009-3084(02)00021-X. 
  • Carter CA, Kane CJ (2005). "Therapeutic potential of natural compounds that regulate the activity of protein kinase C". Curr. Med. Chem. 11 (21): 2883–902. PMID 15544481. doi:10.2174/0929867043364090.